Experiment


Biarc J,Chalkley RJ,Burlingame AL,Bradshaw RA. The Journal of biological chemistry. 2013-June. Vol 288. 16606-18. [pubmed]

A PDGFR (extracellular region) was fused to the rat TRKA transmembrane and intracellular region to create a receptor tyrosine chimera with TRKA-competent signaling that is responsive to PDGF. Then intracellular tyrosine positions, Y490 and Y785 were mutated to isolate the signaling roles of each. Phosphoproteomic measurements were done using SILAC and LC-MS/MS.

Cell PC12
Stimulus PDGF

2017-02-16 15:14:41

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