[MG-132 treatment set] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles


Experiment Name
[MG-132 treatment set] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles
Citation
Wagner SA,Beli P,Weinert BT,Nielsen ML,Cox J,Mann M,Choudhary C. Molecular & cellular proteomics : MCP. 2011-October. Vol 10. 1-13. [pubmed]
Description
Identification of ubiquitylation sites in HEK-293T and MV-411 cells using di-Gly-lysine specific antibody enrichment and mass spectrometry -- The quantitative measurements (SILAC) of ubiquitylation in response to MG-132 treatment (proteosome inhibition for 4 hours compared to DMSO control).
Conditions
Cell
MV4-11
Drug
MG-132
Date Loaded
October 14 2013

Experiment Data Summary

Proteins
1557
Measured Peptides
2729
Modification Sites
2781
Rejected Peptides
256
By Residue By Modification Type By Species
K 2781
Ubiquitination 2781
homo sapiens 2777
bos taurus 4

Peptide Sequence Profile